The aim of the study was to find out the effect of circuit training on selected physical fitness variables among college level women Kho-kho players. To achieve the purpose of the study thirty women Kho-kho players were selected from various departments of Bharathidasan University, Tiruchirappalli. The selected subjects age range between 21 to 23 years. The selected subjects randomly divided in to two equal groups, group I experimental group, group II control group. Each group consists of 15 subjects. Experimental Group I undergone to circuit training for 6 weeks, 6 days per week under training in the morning secessions only. Group II (control group) does not have any specific training other than the regular routine activities. The physical fitness variables such as speed, agility and endurance have been selected as depended variables. The data collected from the two groups prior to and post experimentation were statistically analyzed by analysis of covariance (ANCOVA). The level of significance was fixed at 0.05 of confidence for all the cases. The experimental groups had significant improvement on all the selected physical fitness variables when comparing to the control group.