Topic: Immediate effect of Dynamic soft tissue mobilization verses Novel mobilization technique on hamstring flexibility in college students of age 18 to 25 years- a comparative study. Background: Hamstring tightness is a common musculoskeletal condition with an incidence of 80% in college students [1]. Poor hamstrings flexibility has been associated with low back pain in cross-sectional studies in both adolescents and adults. Poor hamstring flexibility is also considered a risk factor for the development of patellofemoral pain and hamstring strain injury [3] Without proper hamstring flexibility, individuals will not be able to perform simple daily activities which require extending at the hip or bending at the knee [4]. Novel’s Technique includes mulligan traction straight leg raise combined with post-isometric relaxation applied to the hip [6]. Dynamic soft tissue mobilization (DSTM) is a technique that combines with the delivery of manual treatment, such as joint and soft tissue positioning and movements (passive or active) involving concentric or eccentric muscle activity [8]. Objective: To compare the immediate effect of dynamic soft tissue mobilization technique and novel mobilization technique on hamstring flexibility in college students using Sit and Reach test. Methodology: Ethical clearance was obtained from the institution. Informed consent was taken from the participants. Participants were screened according to inclusion and exclusion criteria. Purpose of study and procedure was explained to participants. Participants were divided into two group by odd and even method. Sit and Reach test was taken prior and at the end of intervention. Group A: Receiving Novel Mobilization Technique. Group B: Receiving Dynamic soft tissue mobilization Result: Total 60 students of age group 18-25 years, with Hamstring tightness volunteered to participate in the study and have completed the program. Pre and post analysis was done within group using paired t test which showed significant results and unpaired test was done inter-group which also showed significant results and p value, mean and mean of difference was calculated. Results from the statistical analysis were tabulated and presented in graphical formats for better understanding and easier interpretations. Conclusion: In this study, pre-treatment assessment and post treatment assessment showed significant results on outcome measures, Sit and Reach Test, while increasing flexibility of the hamstring muscle of students. Both the Novel’s and Dynamic Soft Tissue Mobilization ae individually effective in increasing the flexibility, but, a comparison between the groups produced a significant difference of 0.5 which shows that Dynamic Soft Tissue Mobilization has a clinical and statistical advantage than Novel’s Mobilization Technique for improving Hamstring flexibility in college students.