Background: In recent times (21st century), the case of doping in Ethiopian athletes has become a series issue, especially among beginner runners. And also, doping is not the only issue in sports. Still, also it is a concern of society, given that doping is a demanding issue that must be dealt with in the education of future sports science professionals. This study aimed to assess the doping perception among sports science university students in Ethiopia concerning their sports participation background. Methods: A cross-sectional design of the study was functional. All the students (N=736) were part of the sample population due to their small numbers from each targeted governmental university with applied whole or census sample technique. A self-constructed questionnaire was prepared precisely for this study to manage 736 sports science university students. The Independents sample t-test was active to analyze the doping perception of students. The researcher also carried out descriptive statistics (mean, standard deviation, frequency, and percentage) in addition to inferential statistics. Results: There was a significant variance (P<.0.05) between the sports science students regarding their experience or background in sports participation. The result shows that the sports science university students with sports participation experience or knowledge demonstrated better doping perception than those with no sports participation experience or background. Conclusions: It is significant to deliver extra chances for proper education for university sports science students on doping, given concentration, and attending on the topic looks valuable. Besides this, education about doping for sports science, students would be as actual against doping as educating an athlete. Moreover, short-term training should give to graduate students of sports science with the collaboration of ETH-ADA. As well as, ETH-NADA and universities should have to take agreement in between to work together, address the students’ perception gap, and implement doping-free sports in the country.