The purpose of the study was to compare the aggression level between male and female Football, Handball and Hockey players. To achieve the purpose of the study, 150 Male and 150 Female college level players consist of 50 players each from Football, Handball and Hockey players discipline were randomly selected from various part of Tamil Nadu, whose ages ranged between 15 and 20 years were selected at random as subjects. The subject’s Aggression level was assessed by the Smith standardized questionnaire. The selected variable was analysed by using 2 × 3 factorial ANOVA, two-way analysis of variance and result reveals that there was a significant difference among male and female Football, Handball and Hockey players on aggression and also found significant difference between Football, Handball and Hockey players.